Featured in PASCO’s College Biology Instructor Guide

College Biology Instructor Guide

(PS-3800A)

College Instructor edition with 26 experiments covering introductory and general biology topics.

Enzyme Catalysis

Lab Summary

Use an oxygen gas sensor to measure oxygen gas production resulting from the decomposition of hydrogen peroxide under six conditions.

Theory

In this lab, students learn about the enzyme catalase and how factors like temperature and pH affect its activity. Students:

  • Observe the conversion of hydrogen peroxide to water and oxygen gas by the enzyme catalase.
  • Measure the amount of oxygen generated and calculate the rate of the enzyme-catalyzed reaction.
  • Explore the effects of pH and temperature on the rate of the enzyme-catalyzed reaction.

Downloads

  • Enzyme Activity (Teacher and Student) (481 KB – PDF)
  • Method

    Students will gain experience conducting the following procedures:

    • Measuring and analyzing oxygen gas production as the result of the decomposition of hydrogen peroxide under six conditions: spontaneously, in the presence of the enzyme catalase, increased environmental pH, decreased environmental pH, increased environmental temperature, and decreased environmental temperature.
    • Calculating the rate of oxygen production for each data run.

    Sensors / Equipment Used:

    PASPORT Oxygen Gas Sensor

    (PS-2126A)

    The Oxygen Gas Sensor accurately measures O2 concentration in the atmosphere or in enclosed spaces. Study animal, plant or cellular respiration, air quality, O2 production from chemical reactions and more.

    Materials Used:

    1.0 M Hydrochloric acid (HCl) solution - 10 mL
    1.0 M Sodium hydroxide (NaOH) solution-  10 mL
    Activated yeast suspension, boiled - 10 mL
    Activated yeast suspension, chilled - 10 mL
    Activated yeast suspension, room temperature - 30 mL
    Graduated cylinder 10-mL 
    Graduated cylinder 25-mL 
    Hydrogen peroxide, 1.5% - 120 mL
    Saltine cracker - 1 per student
    Sampling bottle (with sensor) 

    The Associated Bundle:

    College Biology Bundle

    (PS-2926B)

    A complete probeware bundle designed for PASCO's College Biology curriculum

    Interface Required

    This experiment may require software and an interface for data collection.