Featured in PASCO’s College Biology Instructor Guide
College Instructor edition with 26 experiments covering introductory and general biology topics.
pH on Amylase Activity
Use a water quality sensor and PASPORT colorimeter to measure the effects of pH on bacterial amylase activity.
In this activity, students investigate the concept that enzymes function within a range of pH. During the lab, students find the pH at which the enzyme bacterial amylase functions optimally. Students:
- Observe the effects of bacterial amylase on a starch solution.
- Explore the effects of pH on the rate of enzyme activity using six buffers of unknown pH.
- Determine the optimal pH for bacterial amylase activity using qualitative and quantitative data.
Students will gain experience conducting the following procedures:
- Use the colorimeter to test the effects of pH on bacterial amylase activity.
- Use qualitative and quantitative data to find the optimal pH for bacterial amylase activity. pH will be varied using six buffers of unknown pH, varying from approximately 3.0 to 8.0.
Sensors / Equipment Used:
Measures temperature, conductivity, pH/ISE/ORP and dissolved oxygen. It is specifically designed for convenience in conducting water quality lab experiments. For use with PASPORT Interfaces.
Our PASPORT Colorimeter records all 4 wavelengths simultaneously--no need to guess at wavelength to get a quality measurement.
0.1 M Hydrochloric acid (HCl) solution - 30 mL
30% Starch solution - 30 mL
Bacterial Amylase solution - 4 mL
Cuvettes (with colorimeter) - 7
Disposable graduated pipets
Distilled water - 200 mL
Graduated cylinder, 10-mL
Lugol's Iodine (IKI) solution - 30 mL
Six buffers of varying pH (pH 3.0, 4.0, 5.0, 6.0, 7.0 and 8.0) - 5 mL each
Small beakers or plastic cups - 19
Standard buffer (pH 10) - 25 mL
Standard buffer (pH 4) - 25 mL
Wash bottle with distilled water
The Associated Bundle:
A complete probeware bundle designed for PASCO's College Biology curriculum