Featured in PASCO’s College Biology Instructor Guide
College Instructor edition with 26 experiments covering introductory and general biology topics.
Use a water quality sensor and PASPORT colorimeter to measure the effects of pH on bacterial amylase activity.
In this activity, students investigate the concept that enzymes function within a range of pH. During the lab, students find the pH at which the enzyme bacterial amylase functions optimally. Students:
Students will gain experience conducting the following procedures:
0.1 M Hydrochloric acid (HCl) solution - 30 mL
30% Starch solution - 30 mL
Bacterial Amylase solution - 4 mL
Cuvettes (with colorimeter) - 7
Disposable graduated pipets
Distilled water - 200 mL
Graduated cylinder, 10-mL
Lugol's Iodine (IKI) solution - 30 mL
Six buffers of varying pH (pH 3.0, 4.0, 5.0, 6.0, 7.0 and 8.0) - 5 mL each
Small beakers or plastic cups - 19
Standard buffer (pH 10) - 25 mL
Standard buffer (pH 4) - 25 mL
Wash bottle with distilled water
This experiment may require software and an interface for data collection.