Featured in PASCO’s College Chemistry Instructor Guide

College Chemistry Instructor Guide

(PS-3803A)

College Instructor edition with 36 experiments covering introductory and general chemistry topics.

Colorimetric Analysis

Lab Summary

Use a colorimeter to learn how the factors of concentration and path length affect the absorbance of a colored solution.

Theory

Students learn how the factors of concentration and path length affect the absorbance of a colored solution.

Method

Students gain experience conducting the following procedures:

  • Diluting a copper (II) sulfate solution of known concentration in order to create five calibration standards.
  • Creating a calibration curve of the absorbance of 660 nanometers of light versus the concentration of the five CuSO4 standards.
  • Determining the concentration of a sixth copper(II) sulfate solution using the calibration curve and the equation of the slope of the best-fit line of the calibration curve.

Sensors / Equipment Used:

PASPORT Colorimeter Sensor

(PS-2121)

Our PASPORT Colorimeter records all 4 wavelengths simultaneously--no need to guess at wavelength to get a quality measurement.

Materials Used:

Glass cuvette with cap
Beakers, 100-mL - 2
Test tubes, large - 6
Test tube rack 
Graduated cylinder, 50-mL
Pipet with 10-mL 
Pipet bulb 
Glass stirring rod 
0.40 M copper(II) sulfate (CuSO4) - 30 mL
Distilled water - 30 mL
Marking pen 
Wash bottle with distilled water

The Associated Bundle:

Advanced Chemistry Standard Sensor Bundle

(PS-2928A)

Contains the probeware for completing experiments in the College Chemistry lab manual.

Interface Required

This experiment may require software and an interface for data collection.