Featured in PASCO’s College Biology Instructor Guide
College Instructor edition with 26 experiments covering introductory and general biology topics.
Use a colorimeter to explore the effects of habitat size, nutrient availability, initial population density and temperature on the growth rate of E.coli bacteria.
Students use the bacteria E. coli as a model for studying population growth, growth curves, and factors affecting growth.
Students gain experience conducting the following procedures:
- Comparing the growth rate (r) of E. coli populations in different sized chambers.
- Comparing r in E. coli populations with different nutrient availability.
- Comparing r in E. coli populations with different initial population densities.
- Comparing r in E. coli populations incubated at different temperatures.
Sensors / Equipment Used:
Our PASPORT Colorimeter records all 4 wavelengths simultaneously--no need to guess at wavelength to get a quality measurement.
Culture vessels, 15-mL - 3
Culture vessels, 250-mL - 3
Culture vessels, 50-mL - 3
Cuvettes (with colorimeter) - 4
Luria-Bertani (LB) broth - 40 mL
Overnight (O/N) culture of E.coli in a 15- mL culture tube - 4 mL
Shaking Incubator (optional) - 1 per class
Squirt bottle with 10% bleach
Sterile transfer or Pasteur pipets, toothpicks, or inoculating loop
Sterile water - 10 mL
The Associated Bundle:
A complete probeware bundle designed for PASCO's College Biology curriculum