Featured in PASCO’s College Biology Instructor Guide
College Instructor edition with 26 experiments covering introductory and general biology topics.
Pigments & Photosynthesis
Use a colorimeter to determine the rate of photosynthesis in a suspension of chloroplasts.
Students learn how to separate plant pigments using paper chromatography and how to measure the rate of photosynthesis in chloroplast suspensions.
Students gain experience conducting the following procedures:
- Separating plant pigments based on their solubility using paper chromatography.
- Determining the rate of photosynthesis in a suspension of chloroplasts using a colorimeter and the reduction of 2,6-dichlorophenolindophenol (DPIP).
Sensors / Equipment Used:
Our PASPORT Colorimeter records all 4 wavelengths simultaneously--no need to guess at wavelength to get a quality measurement.
#1 Whatman Chromatography paper - 10 to 12 cm
0.1 M phosphate buffer - 4 mL
Chloroplast suspension - 2 mL
Chromatography solvent - 5 mL
Cuvettes (with colorimeter) - 5
Distilled water - 13 mL
DPIP in small amber bottle - 3 mL
Floodlight, 100 watt
Glass jar, 10-12 cm tall
Graduated disposable pipet 1-mL - 2
Heat sink (large beaker or flask filled with water)
Ice and water - 1 L
Roll of aluminum foil - 1 per class
Spinach - 1 leaf
The Associated Bundle:
A complete probeware bundle designed for PASCO's College Biology curriculum