Featured in PASCO’s College Biology Instructor Guide
College Instructor edition with 26 experiments covering introductory and general biology topics.
Use a colorimeter to determine the rate of photosynthesis in a suspension of chloroplasts.
Students learn how to separate plant pigments using paper chromatography and how to measure the rate of photosynthesis in chloroplast suspensions.
Students gain experience conducting the following procedures:
#1 Whatman Chromatography paper - 10 to 12 cm
0.1 M phosphate buffer - 4 mL
Chloroplast suspension - 2 mL
Chromatography solvent - 5 mL
Cuvettes (with colorimeter) - 5
Distilled water - 13 mL
DPIP in small amber bottle - 3 mL
Floodlight, 100 watt
Glass jar, 10-12 cm tall
Graduated disposable pipet 1-mL - 2
Heat sink (large beaker or flask filled with water)
Ice and water - 1 L
Roll of aluminum foil - 1 per class
Spinach - 1 leaf
This experiment may require software and an interface for data collection.