Featured in PASCO’s College Biology Instructor Guide

College Biology Instructor Guide

(PS-3800A)

College Instructor edition with 26 experiments covering introductory and general biology topics.

Genetics of Organisms

Lab Summary

Use the common fruit fly, Drosophila melanogaster, to create and statistically analyze genetic crosses.

Theory

This lab allows students to use the common fruit fly, Drosophila melanogaster, to perform genetic (monohybrid, dihybrid, and sex-linked) crosses. This lab provides an excellent opportunity to work with and manipulate live organisms in a laboratory setting. During this investigation, students explore:

  • The relationship between the genotype and phenotype of wild-type and mutant fruit flies.
  • The relationship between F1 and F2 generations in monohybrid, dihybrid, and sex-linked crosses.
  • The life-cycle of a metamorphosing insect.
  • Statistical analysis of genetic crosses.

Method

Students gain experience conducting the following procedures:

  • Culturing and anesthetizing fruit flies.
  • Determining the sex of fruit flies.
  • Observing phenotype of wild-type and mutant fruit flies.
  • Statistically analyzing results using the chi-square test.

Sensors / Equipment Used:

kena Digital Microscope

(SE-7236)

USB connected and powered for experiments in the classroom or the field.

Materials Used:

Anesthetizing material 
Culture vial 
Culture vial label or labeling tape 
Fly morgue 
Foam plug 
Index cards - 2
Instant Drosophila culturing medium - 1 to 2 g
Mutant flies (autosomal monohybrid F1 cross) - 1 vial per class
Mutant flies (autosomal recessive dihybrid F1 cross) - 1 vial per class
Mutant flies (sex-linked F1 cross) - 1 vial per class
Petri dish 
Screens 
Small, thin, camel hair paint brush 
Wild type flies - 3 vials per class

The Associated Bundle:

College Biology Bundle

(PS-2926B)

A complete probeware bundle designed for PASCO's College Biology curriculum

Interface Required

This experiment may require software and an interface for data collection.