Featured in PASCO’s College Biology Instructor Guide
College Instructor edition with 26 experiments covering introductory and general biology topics.
Use restriction endonuclease digests and agarose gel electrophoresis to diagnose an inherited mitochondrial disease.
**Requires Mitochondrial DNA Kit (BP-6946)**
This mitochondrial genetics lab uses molecular techniques to answer a question about how a mitochondrial disease is inherited and diagnosed. The lab consists of two essential activities: pedigree construction from patient case histories, and molecular analysis of a point mutation in the mitochondrial genome. Students:
Students will gain experience conducting the following procedures:
1X TBE buffer - 300 to 400 mL
Adjustable micropipet (2 to 20 ul)
Agarose or pre-cast 1.5% agarose gels
Disposable gloves - 1 pair
Electronic Balance - 1 per class
Horizontal gel electrophoresis box and power supply
Loading buffer/dye (in 1.5-mL microcentrifuge tube) - 8 uL
Methylene blue DNA stain - 100 mL
Microcentrifuge (optional) - 1 per class
Microcentrifuge tube, 1.5-mL - 6
Molecular weight marker (in 1.5-mL microcentrifuge tube) - 12 uL
Sterile, disposable micropipet tips - 10
This experiment may require software and an interface for data collection.