Featured in PASCO’s College Biology Instructor Guide

College Biology Instructor Guide

(PS-3800A)

College Instructor edition with 26 experiments covering introductory and general biology topics.

Genetics & Biotech

Lab Summary

Use restriction endonuclease digests and agarose gel electrophoresis to diagnose an inherited mitochondrial disease.

**Requires Mitochondrial DNA Kit (BP-6946)**

Theory

This mitochondrial genetics lab uses molecular techniques to answer a question about how a mitochondrial disease is inherited and diagnosed. The lab consists of two essential activities: pedigree construction from patient case histories, and molecular analysis of a point mutation in the mitochondrial genome. Students:

  • Use restriction endonuclease digests and agarose gel electrophoresis to diagnose an inherited mitochondrial disease.
  • Construct pedigrees from patient case histories and correlate patterns of inheritance with molecular diagnosis.
  • Investigate the relationship between mitochondrial DNA and nuclear DNA in the synthesis of the protein complexes of the electron transport chain.

Method

Students will gain experience conducting the following procedures:

  • Analyzing the products of a restriction digest by gel electrophoresis to detect a point mutation in the mitochondrial genome.
  • Constructing pedigrees from patient family histories to determine the pattern of inheritance of a genetic disease.
  • Gathering evidence from both the molecular diagnosis and pedigree construction to confirm the diagnosis of a genetic disease.

Sensors / Equipment Used:

Mitochondrial Genetics Kit

(BP-6946)

Mitochondrial Genetics Kit for studying genetics and biotechnology.

Materials Used:

1X TBE buffer - 300 to 400 mL
Adjustable micropipet (2 to 20 ul) 
Agarose or pre-cast 1.5% agarose gels 
Disposable gloves - 1 pair
Electronic Balance - 1 per class
Horizontal gel electrophoresis box and power supply
Loading buffer/dye (in 1.5-mL microcentrifuge tube) - 8 uL
Methylene blue DNA stain - 100 mL
Microcentrifuge (optional) - 1 per class
Microcentrifuge tube, 1.5-mL - 6
Molecular weight marker (in 1.5-mL microcentrifuge tube) - 12 uL
Staining tray 
Sterile, disposable micropipet tips - 10
Waste receptacle 

The Associated Bundle:

College Biology Bundle

(PS-2926B)

A complete probeware bundle designed for PASCO's College Biology curriculum

Interface Required

This experiment may require software and an interface for data collection.