Featured in PASCO’s College Biology Instructor Guide
College Instructor edition with 26 experiments covering introductory and general biology topics.
Use the tools of biotechnology to transform competent E.coli cells and then select for antibiotic resistance.
In this lab, students transform E. coli bacteria and screen them for the presence of antibiotic resistance. Students:
Students will gain experience conducting the following procedures:
Calcium chloride solution - 500 ul
Clear labeling tape
E.Coli starter plate
LB/Amp agar plate - 2
Luria Bertani (LB) agar plate - 2
Micropipettor with sterile tips
pAmp plasmid - 10 ul
Sterile test tubes, 15-mL - 2
Sterile, glass spreading rod
Water bath (42ºC) - 1 per class
Wax labeling pencil
Wire inoculating loop
This experiment may require software and an interface for data collection.