Featured in PASCO’s College Biology Instructor Guide

College Biology Instructor Guide


College Instructor edition with 26 experiments covering introductory and general biology topics.

Aerobic Respiration of Yeast

Lab Summary

Use a water quality sensor to measure the effects of temperature on the dissolved oxygen concentration of active yeast cultures.


In this activity, students investigate how to:

  • Calculate the rate of oxygen consumption of yeast during aerobic cellular respiration.
  • Use rate of oxygen consumption as a measure of the rate of aerobic cellular respiration in yeast at varying temperatures.
  • Identify a control group, and independent, dependent, and constant variables in an experiment.


Students will gain experience conducting the following procedures

  • Measuring the dissolved oxygen concentration of a sugar solution before and after adding an activated yeast solution.
  • Measuring the dissolved oxygen concentration of diluted sugar solutions at varying temperatures. They will use the temperature probe before and after adding the activated yeast solution.

Sensors / Equipment Used:

PASPORT Water Quality Sensor


Measures temperature, conductivity, pH/ISE/ORP and dissolved oxygen. It is specifically designed for convenience in conducting water quality lab experiments. For use with PASPORT Interfaces.

Materials Used:

Activated yeast solution - 45 mL
Beaker, 250-mL - 3
Distilled or deionized water - 1 L
Electronic Balance - 1 per class
Graduated cylinder, 100-mL 
Hot Plate 
Ice bath, 1 L beaker filled with ice water 
Labeling marker 
Labeling tape 
Stirring rod 
Sugar - 30 g
Weighing paper - 3

The Associated Bundle:

College Biology Bundle


A complete probeware bundle designed for PASCO's College Biology curriculum

Interface Required

This experiment may require software and an interface for data collection.