Featured in PASCO’s Biology through Inquiry Teacher Guide

Biology through Inquiry Teacher Guide


Features 25 inquiry based labs covering the fundamental concepts of cell biology, ecology and physiology.

Organisms & pH

Lab Summary

Use a pH sensor to determine how effective various substances are at buffering large changes in pH.


Students explore pH and its impact on biological organisms by:

  • Determining the importance of pH and the use of chemical buffers to organisms.
  • Indicating the effects of pH on the function of biological molecules and other various common compounds.



Students gain experience by conducting the following procedures:

  • Test changes in pH after adding a variety of substances to a solution.
  • Test the effects of buffers on the pH of substances.
  • Analyze the results and apply to living systems and organisms.

Sensors / Equipment Used:



Has a wide measurement range of 0 to 14 pH. Has a resolution of 0.01 pH. For use with PASPORT Interfaces.

Materials Used:

Beaker (6), 50-mL

Beaker, 250-mL

Erlenmeyer flask (2), 1-L

Graduated cylinder, 10-mL

Disposable pipets (2)

Detergent solution

Lemon juice

Distilled water

Liver suspension

Buffer solution

Your Bundle Options:

Option 1:

Biology Starter Sensor Bundle


A sensor bundle specifically designed to get you started using probeware in your biology lab. Also: perform 10 of the 24 Lab Activities from Biology with Inquiry.

Option 2:

Biology Standard Sensor Bundle


The Biology Standard Sensor Bundle includes a photosynthesis tank and the sensors required to perform 19 of the 24 Lab Activities in the Biology through Inquiry manual.

Interface Required

This experiment may require software and an interface for data collection.