Featured in PASCO’s Biology through Inquiry Teacher Guide

Biology through Inquiry Teacher Guide

(PS-2870C)

Features 25 inquiry based labs covering the fundamental concepts of cell biology, ecology and physiology.

Cell Respiration in Yeast

Lab Summary

Use a dissolved oxygen sensor to measure the dissolved oxygen concentration in yeast solutions in the presence and absence of sugar. Calculate the rate of oxygen consumption of yeast during aerobic cellular
respiration at different temperatures.

Theory

Guide students to investigate how to measure the dissolved oxygen concentration in yeast solutions in the presence and absence of sugar at different temperatures.

Method

Students will gain experience conducting the following procedures:

  • Use a dissolved oxygen sensor to measure the concentration of dissolved oxygen in a dilute sugar solution.
  • Measure the dissolved oxygen concentration of a sugar solution before and after adding an activated yeast solution.
  • Measuring the dissolved oxygen concentration of dilute sugar solutions at varying temperatures, using the temperature sensor, before and after adding activated yeast solution.

Sensors / Equipment Used:

PASPORT Dissolved Oxygen Sensor

(PS-2108)

Provides real-time, aqueous oxygen concentration measurements. For use with PASPORT Interfaces.

PASPORT Fast Response Temperature Probe (3 pack)

(PS-2135)

Allows students to see changes in temperature they could never see before. For use with PASPORT and ScienceWorkshop Interfaces.

OHAUS Scout SKX Balance 200g

(SE-8823A)

Combines range, resolution and low cost, making it ideal for the student science lab. 200 g version.

Hot Plate

(SE-8830)

This compact and durable hot plate can reach up to 400 °C in minutes. The cast aluminum top plate provides an even heating surface, and it even has a built in support rod holder.

Materials Used:

Beaker (3), 250-mL

Graduated cylinder

Sugar, 30 g

Activated yeast solution, 45 mL

Distilled water, 1 L

ICe bath (1-L beaker filled with ice)

Weighing papers (3)

Stirring rod

Labeling tape

Marker

The Associated Bundle:

Biology Standard Sensor Bundle

(PS-2925A)

The Biology Standard Sensor Bundle includes a photosynthesis tank and the sensors required to perform 19 of the 24 Lab Activities in the Biology through Inquiry manual.

Interface Required

This experiment may require software and an interface for data collection.