Advanced Environmental and Earth Sciences Teacher Guide

(PS-2979)

Teacher manual for Advanced Environmental and Earth Sciences labs. Has both the printed version and a flash drive with teacher tips, a PDF of teacher version and editable Word student version.

Yeast Respiration

Lab Summary

Use a dissolved oxygen sensor, a carbon dioxide sensor, and the EcoChamber™ to analyze aerobic and anaerobic respiration by yeast cells.

Theory

Students use yeast cells as a simple model for studying both aerobic and anaerobic cellular respiration. As part of this investigation, they:

  • Analyze evidence of aerobic and anaerobic respiration by yeast cells.
  • Discuss the role of cellular respiration in the carbon and oxygen cycles.
  • Explain the role yeast cells play in scientific exploration.

Method

Students gain experience conducting the following procedures:

  • Creating a closed-system for collecting and interpreting data collected by CO2 gas and dissolved oxygen sensors.
  • Using a microscope to observe and describe yeast cells.

Sensors / Equipment Used:

Advanced Water Quality Sensor

(PS-2230)

MultiMeasure sensor that incorporates pH, conductivity, temperature, and the optical dissolved oxygen probe.

PASPORT Carbon Dioxide Gas Sensor

(PS-2110)

The PASPORT Carbon Dioxide Gas Sensor measures carbon dioxide concentration CO2 (parts per million) in gases such as air. Ideal for studies of photosynthesis, respiration, fermentation and more.

EcoChamber

(ME-6667)

A single acrylic chamber designed to integrate sensors for controlled-environment experiments such as fermentation and plant or cricket respiration.

Materials Used:

Activated baker's yeast, 7-g packet 
Beaker, 1 L 
Graduated cylinder, 500-mL or 1-L 
Hot plate with magnetic stirrer and stir bar
Magnetic stir plate with stir bar 
Microscope slide and cover slip 
Microscope with magnification to 400x
Pipet, disposable 
Sugar - 100 g
Water - 1 L

The Associated Bundle:

Advanced Environmental and Earth Science Standard Sensor Bundle

(PS-2916A)

Standard bundle for Advanced Environmental and Earth Sciences Teacher Guide,

Interface Required

This experiment may require software and an interface for data collection.