Teacher manual for Advanced Environmental and Earth Sciences labs. Has both the printed version and a flash drive with teacher tips, a PDF of teacher version and editable Word student version.
Toxicology Using Yeast
Use a carbon dioxide gas sensor and a pH sensor to evaluate the role of pH in toxicity and the role of cell culture in toxicology studies.
Students demonstrate how yeast cells can serve as simple models to assess chemical hazards. They also:
- Evaluate the role of pH in toxicity.
- Evaluate the role of cell culture in toxicology studies.
Students determine the relative toxicity of half-strength bleach and full-strength vinegar using a yeast cell culture system. They gain experience completing the following procedures:
- Conducting toxicology studies of yeast cell cultures in a closed system, measuring the carbon dioxide gas level as the indicator of toxicity, and correlating pH with toxicity.
- Extrapolating the median effective dose (ED50) for half-strength bleach and full-strength vinegar from experimental data.
Sensors / Equipment Used:
The PASPORT Carbon Dioxide Gas Sensor measures carbon dioxide concentration CO2 (parts per million) in gases such as air. Ideal for studies of photosynthesis, respiration, fermentation and more.
MultiMeasure sensor that incorporates pH, conductivity, temperature, and the optical dissolved oxygen probe.
A single polycarbonate chamber designed to integrate sensors for controlled-environment experiments such as fermentation and plant or cricket respiration.
Beaker, 100-mL (for vinegar)
Beaker, glass, 2-L
Erlenmeyer flask, 125-mL
Graduated cylinder, 1-L or 500-mL
Graduated cylinder, 25-mL or 10-mL
Household bleach, half-strength - 50 mL
Magnetic stir plate and stir bar
Rapid-rise activated baker's yeast (7-g packet)
Rubber stopper for Erlenmeyer flask
Sugar - 100 g
Water - 1 L
White vinegar - 50 mL
The Associated Bundle:
Standard bundle for Advanced Environmental and Earth Sciences Teacher Guide,