Teacher manual for Advanced Environmental and Earth Sciences labs. Has both the printed version and a flash drive with teacher tips, a PDF of teacher version and editable Word student version.
Use a carbon dioxide gas sensor and a pH sensor to evaluate the role of pH in toxicity and the role of cell culture in toxicology studies.
Students demonstrate how yeast cells can serve as simple models to assess chemical hazards. They also:
Students determine the relative toxicity of half-strength bleach and full-strength vinegar using a yeast cell culture system. They gain experience completing the following procedures:
Beaker, 100-mL (for vinegar)
Beaker, glass, 2-L
Erlenmeyer flask, 125-mL
Graduated cylinder, 1-L or 500-mL
Graduated cylinder, 25-mL or 10-mL
Household bleach, half-strength - 50 mL
Magnetic stir plate and stir bar
Rapid-rise activated baker's yeast (7-g packet)
Rubber stopper for Erlenmeyer flask
Sugar - 100 g
Water - 1 L
White vinegar - 50 mL
This experiment may require software and an interface for data collection.