Featured in PASCO’s Advanced Biology through Inquiry Teacher Guide

Advanced Biology through Inquiry Teacher Guide

(PS-2852)

This manual features 22 challenging, standards-based, inquiry lab activities for AP, IB, and honors programs.

Understanding Inherited Mitochondrial Disorders

Lab Summary

Students use pedigree analysis and DNA analysis (electrophoresis) to confirm or refute the initial diagnosis of MELAS for two patients.

Theory

Students use pedigree analysis and DNA analysis to confirm or refute the initial diagnosis of MELAS for two patients. MELAS and certain other genetic mitochondrial disorders are unusual in their inheritance: the mutation is passed along maternally only. This maternal inheritance, combined with the complexity in symptoms associated with the disorder, provides a case study that requires students to think critically and evaluate multiple lines of evidence.

Method

The two investigations, “Pedigree Investigation” and “DNA Investigation,” both relate to the same two patients. Either investigation can be performed first or students can complete the investigations simultaneously. Based on the length of your class periods, your students' prior knowledge, and your own pedagogical preference, you can determine how best to organize the investigations. In addition to the time required for the investigations, allow time for a discussion of the background information regarding the mitochondrial genome and MELAS. (Photocopy the supplement at the end of this document for students.)

Materials Used:

Edvotek

The Associated Bundle:

Advanced Biology Standard Sensor Bundle

(PS-2851)

Contains an EcoChamber, a Metabolism Chamber and all the probeware needed to perform the activities in the Advanced Biology through Inquiry lab manual.

Interface Required

This experiment may require software and an interface for data collection.