Featured in PASCO’s Advanced Biology through Inquiry Teacher Guide

Advanced Biology through Inquiry Teacher Guide

(PS-2852)

This manual features 22 challenging, standards-based, inquiry lab activities for AP, IB, and honors programs.

Sickle Cell Gene Detection

Lab Summary

Students use electrophoresis to analyze DNA samples from a child and the child’s parents to determine if the child has inherited a mutation in the gene for hemoglobin B.

Theory

Many genetic disorders can be detected and diagnosed by a method employing polymerase chain reaction (PCR), restriction enzymes, and electrophoresis. Genetic tests often rely on digesting DNA with a restriction enzyme, which can cut within a normal gene sequence but not within the mutated sequence, resulting in different lengths of DNA. Electrophoresis separates DNA fragments based on the sizes of the fragments.

Method

In this lab, students use electrophoresis to analyze DNA samples (previously treated with a restriction enzyme) from a child and the child’s parents to determine if the child has inherited a mutation in the gene for hemoglobin B (HBB). The mutation causes sickle cell anemia, a common form of sickle cell disease, in homozygous individuals.

Materials Used:

Edvotek

The Associated Bundle:

Advanced Biology Standard Sensor Bundle

(PS-2851)

Contains an EcoChamber, a Metabolism Chamber and all the probeware needed to perform the activities in the Advanced Biology through Inquiry lab manual.

Interface Required

This experiment may require software and an interface for data collection.