Transformation of E. coli with Green Fluorescent Protein (GFP) Kit - BP-6948

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Overview

Transformed cells take up a plasmid containing the GFP gene. The GFP gene is isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light, but will fluoresce brightly when exposed to long wave UV light.

All you need: automatic micropipet (5-50 µl) and tips, two waterbaths (37ºC and 42ºC), thermometer, incubation oven (37ºC), pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light

Storage: Some Components Require Refrigerator and Freezer Storage

  • A note on availability: This product may not be available directly from PASCO or your local PASCO partner in the following geography: Australia. Please contact us with your interest and we will advise you of availability or refer you to another source.

Includes

  • Student Lab Activity with Instructions
  • BactoBeads
  • E. coli GFP Host
  • Supercoiled pFluoroGreen plasmid DNA
  • ampicilin
  • IPTG
  • CaCl2
  • Growth Additive
  • ReadyPour Luria Broth Agar (sterile)
  • Luria Broth Medium for Recovery (sterile)