Transformed cells take up a plasmid containing the GFP gene. The GFP gene is isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light, but will fluoresce brightly when exposed to long wave UV light.
All you need: automatic micropipet (5-50 µl) and tips, two waterbaths (37ºC and 42ºC), thermometer, incubation oven (37ºC), pipet pumps or bulbs, ice, marking pens, bunsen burner, hot plate or microwave, hot gloves, long wave UV light
Storage: Some Components Require Refrigerator and Freezer Storage