Physiology: Metabolism of Yeast in Grape Juice

Metabolism is controlled by enzymes.  Explore what happens to the metabolism of unicellular organisms if their environmental temperature changes or if a chemical inhibitor is added to the solution.

Lab Summary:

Explore the rate of respiration.

Download Free Lab  (PDF, 1.8 MB)

(student version)

Method:

Use a Carbon Dioxide Gas Sensor to compare the production of carbon dioxide gas in a sampling bottle containing yeast and grape juice to the production when a chemical inhibitor is added to the mixture.

Here's What You Need:

Probeware

• PASPORT Carbon Dioxide Gas Sensor (PS-2110)
• PASPORT Extension Cable (PS-2500)
• PASPORT Fast Response Temperature Probe (3 pack) (PS-2135)
• Xplorer GLX Graphing Datalogger (PS-2002)
  (or alternate PASPORT interface -- see other options)

Other Materials

• OHAUS Triple Beam Balance w/o Tare (SE-8723)
• Beaker, 1000 ml (6 Pack) (SE-7288)
• Hot Plate (SE-8767)
• Beaker, 250 mL
• Magnetic Stirrer with Stir Bar
• Pipette
• Mineral Oil, 5 mL
• Sodium Fluoride, Solid, 1 g
• Yeast, Dry, 1 package
• Water, 1 L
• Weighing paper

Lab Manual:

This activity is included in Biology with the Xplorer GLX (PS-2820), complete with Teacher Information pages, student instructions and handouts, and sample data for a total of 20 probeware-based labs. For your convenience, we offer bundles which provide the probeware necessary for the activities in the manual. For more information on these resources and bundles, click here.