Directly measure the activity of yeast during anaerobic respiration.
A complete solution for learning about fermentation and anaerobic respiration
Measure oxygen, carbon dioxide and ethanol rates directly
Study biofuel as an alternative energy
The Teaching Challenge
Traditional fermentation experiments use indirect measurements such as carbon dioxide production as a measure of anaerobic respiration in yeast. PASCO’s solution for the study of yeast fermentation provides a way to directly measure the activity of yeast during anaerobic respiration. Using the PASPORT oxygen, carbon dioxide and ethanol gas sensors, students can gather evidence of fermentation by yeast.
Finding alternative energy sources is an issue of increasing importance in the global community. Scientists all over the world are searching for ways to create clean, renewable energy that will reduce our dependence on non-renewable, petroleum based sources of energy. One of the most attractive choices is the production of biofuels, including ethanol. The study of ethanol and other biofuels are an amazing opportunity to study relevant scientific topics across many content areas.
The PASCO Solution
In this experiment, PASCO’s new EcoChamber is filled with approximately 500 ml of 0.5 M sucrose solution and 1000 mL of yeast solution. The PASPORT oxygen, carbon dioxide and ethanol gas sensors are inserted into the lid of the EcoChamber and all other holes are filled with stoppers to create an airtight environment. To ensure the homogeneity of the solution, the EcoChamber is placed above a magnetic stirrer and the solution is stirred with a stir bar during data collection.
Students will observe the fermentation of sucrose as ethanol concentration increases in the chamber. The data are supported by the simultaneous production of CO2 and consumption of O2. By monitoring the rate of O2 consumption compared to the rate of ethanol production, students can observe the yeast exercise it’s characteristic as a facultative anaerobe and drastically shift from aerobic to anaerobic respiration when O2 becomes scarce.
Solution at a Glance
PASPORT Ethanol Sensor (PS-2194)
PASPORT Oxygen Gas Sensor (PS-2126A)
PASPORT Carbon Dioxide Gas Sensor (PS-2110)
Magnetic Stirrer (SE-7700)
SPARK Science Learning System (PS-2008A)
For more product information and to order products separately, see below.
How It Works
EcoChamber is an acrylic chamber specially designed to accommodate up to three
PASCO sensors. The EcoChamber can be used as a fermentation chamber in yeast
fermentation experiments, a model ecosystem in photosynthesis and respiration
experiments and a temperature regulation chamber when exploring the differences
between endothermic and ectothermic animals. Its sturdy design and custom
molding makes PASCO’s EcoChamber a versatile science learning tool that is easy
to use and easy to clean.
Traditional fermentation experiments use indirect
measurements such as carbon dioxide production as a measure of anaerobic
respiration in yeast. PASCO’s solution for the study of yeast fermentation
provides a way to directly measure the activity of yeast during anaerobic
respiration. Using the PASPORT oxygen, carbon dioxide and ethanol gas sensors,
students can gather evidence of fermentation by yeast.
O2 (ppm), CO2 (ppm), and Ethanol (%) Concentration vs. Time (hours)
SPARKlink is a sensor interface with two ports to accept PASPORT sensors and USB connection to a computer running SPARKvue software. Built in temperature and voltage sensors allow for measurements right out of the box.
USB Connection Cable for the Xplorer GLX and SPARK Science Learning System (PS-2528)