December, 2002, Biology Experiment:

Every Breath I Take

- Purpose
- Background Information
- Equipment & Supplies
- Software & Probeware Set-up
- Experimental Procedure
- Data Analysis
- Conclusions and Extensions




PASPORT CO2 Gas Sensor
(PS-2110)

PASPORT CO2 Gas Sensor

ScienceWorkshop CO2 Gas Sensor
(CI-6561 - Available Jan 2003)

ScienceWorkshop CO2 Gas Sensor

CO2 graphic

Purpose:

Students will use a CO2 Gas Sensor to measure the amount of CO2 in their breath as they exhale, both before and after exercise.

 

Enlarge Diagram

Background Information:

Oxygen is highest on the list of substances required to support human life, followed by water, then food. People may live weeks without food, days without water, but only minutes without breathing. People must breathe constantly, and breathing patterns change depending on the situation, such as during or after exercise. Breathing is the only body function that can be controlled consciously or unconsciously.

Natural breathing automatically maintains a very specific gas ratio of O2 to CO2 in the blood stream; 6.5% CO2 and only 2% O2. Each breath contains 10 times more oxygen and 200 times less carbon dioxide than we need.

It is possible to breathe too much (hyperventilation). When we breathe normally, hemoglobin, the principle carrier of oxygen in the body, remains 98% oxygen (O2) saturated. When we breathe too quickly, we increase O2 saturation slightly but lose too much CO2, which is essential for O2 utilization. When the level of CO2 decreases too much the hemoglobin does not release O2 to the tissues (the Bohr Effect), which causes O2 starvation. The simple remedy is to breathe into a paper bag so exhaled CO2 is re-breathed and the CO2 level in the blood stream is restored.

Hypothesize: Will the CO2 levels of a student’s breath be higher or lower after exercise? Why?

Back to top

Equipment & Supplies:

For each lab group:

Back to top

Software & Probeware Set-up:

  1. Ensure that your USB Link, Xplorer or ScienceWorkshop 500 interface is connected to the computer.

  2. Click on one of the links below to download a pre-configured DataStudio file for this experiment, and then open the file.

    PASPORT users: PS Windows (.zip file) or PS Macintosh (.sit file)

    ScienceWorkshop 500 users: (DataStudio files will be available in January, 2003, when the CO2 Sensor is released.)

    When the file is opened, you should see a Graph Display of CO2 vs. Time, as well as a Digits Display of CO2.

  3. Connect the CO2 Sensor to the Xplorer or USB Link (PASPORT users) or plug the CO2 Sensor into channel A of the 500 Interface (ScienceWorkshop 500 users).

    If you are using the ScienceWorkshop 500 Interface, be sure the interface is turned on and the CO2 Sensor is associated correctly in the Experiment Setup window.

  4. Calibration of the CO2 Sensor is not required for most activities, as the sensor is factory calibrated. Over time, the sensor’s characteristics may change. To restore the sensor to its factory-calibrated state, calibrate in typical outside air conditions (approx. 400 to 450 ppm). For help with calibration procedures, refer to the sensor manual or Quick-Reference card, or access DataStudio’s help menu.

  5. Resize and arrange the display as needed.

Experimental Procedure:

Equipment Setup:

  1. The student should hold the black sensing tube of the CO2 Sensor approximately 2 inches from their mouth.

Data Collection & Recording:

  1. Click the Start button ( ) to begin collecting CO2 data.

  2. Allow the CO2 reading to stabilize for approximately 15 seconds.

  3. Have the student take a deep breath and exhale evenly for about 5 seconds toward the black sensing tube of the sensor.

  4. Wait approximately 30 seconds for the CO2 reading to level off.

  5. Click the Stop button ( ) to end the experiment.

  6. Have the student perform an aerobic exercise (i.e. running in place, jumping jacks…) for several minutes.

  7. Repeat steps 1 thru 5.

Back to top

Data Analysis:

  1. Scale the axes to fit the data using the Scale to Fit button ( ) in the Graph toolbar.

  2. Examine the graph and study the data. Which data run had the highest level of CO2?

Conclusions and Extensions:

  1. How did the level of CO2 differ between the two runs?

  2. Explain why a difference occurred.


Back to top