Cell Biology: Respiration of Germinating Peas

See real-time evidence that germinating seeds are engaged in cellular respiration.

Motion Sensors

See rapid significant differences in CO2 production between germinating and dormant seeds. Enlarge graph.

Lab Summary:

Using a Carbon Dioxide Gas sensor, students will directly observe that germinating seeds produce CO2 gas at a much faster rate than dormant seeds. This experience will help students understand that cellular respiration begins as soon as seeds are exposed to water, which is necessary to support their life processes. After 24 hours, students will observe the seeds for signs of germination and relate their observations to the changes in the CO2 gas concentrations inside the flask.

Download free labs:

(student versions)

Computer-based lab (PDF, 624 KB)

Xplorer GLX-based lab (PDF, 1.3 MB)

Method:

Use the CO2 Gas Sensor to directly measure the CO2 gas produced by germinating and dormant seeds.

Here's What You Need:

Required for computer-based lab:

(See lab download for Xplorer GLX-based lab requirements, which may differ)

Probeware

Other Materials

  • Erlenmeyer Flask, 250-mL
  • Magnifying Glass
  • Protective Gear
  • Paper Towel, 1 sheet
  • Beans or Pea Seeds (40)
  • Water, 100.0 mL

Lab Manual:

The computer-based activity is included in Explorations in Biology (PS-2807). The Xplorer GLX-based lab is included in Biology with the Xplorer GLX (PS-2820). Both manuals are complete with Teacher Information pages, student instructions and handouts, and sample data.

For your convenience, we offer bundles which provide the probeware for the activities in the manual. For more, please see:
Computer-based Biology Lab Manual and Bundles
Xplorer GLX-based Biology Lab Manual and Bundles