PASCO : Biology : Membrane Permeability

Cell Biology: Membrane Permeability

This is where the value statement will be placed. This is where the value statement will be placed.

Place a “cell” containing HCl into a water bath and over time you will see that the pH of the water bath has decreased. This indicates that the membrane is permeable to H + ions.. Enlarge graph.

Lab Summary:

Explore the permeability of a cell-like membrane to hydrogen and hydroxide ions.

Download Free Lab (PDF, 656 KB)

(student version)

Method:

Use a pH Sensor to measure the pH outside of a model cell to determine its permeability to hydroxide and hydrogen ions.

Here's What You Need:

Probeware

Other Materials

Large Base and Support Rod (ME-9355)
Clamp, Buret (SE-9446)
Graduated Cylinder, 50 mL (12 pack) (SE-7289)
Beaker, 250 mL
Binder clip
Magnetic Stirrer and Stir Bar
Wash bottle
Dialysis tubing, 15 cm, 2
Hydrochloric Acid. 1 molar, 15 mL
Sodium Hydroxide, 1 molar, 15 mL
Water, Distilled, 1 L

Lab Manual:

This activity is included in Biology with the Xplorer GLX (PS-2820), complete with Teacher Information pages, student instructions and handouts, and sample data for a total of 20 probeware-based labs. For your convenience, we offer bundles which provide the probeware necessary for the activities in the manual. For more information on these resources and bundles, click here.