Respiration of Germinating Peas
See real-time evidence that germinating seeds are engaged in cellular respiration.
See rapid significant differences in CO2 production between germinating and dormant seeds.
Lab Summary
Using a Carbon Dioxide Gas sensor, students will directly observe that germinating seeds produce CO2 gas at a much faster rate than dormant seeds. This experience will help students understand that cellular respiration begins as soon as seeds are exposed to water, which is necessary to support their life processes.
After 24 hours, students will observe the seeds for signs of germination and relate their observations to the changes in the CO2 gas concentrations inside the flask.
Downloads
- Computer-based lab (PDF, 624 KB) (622 KB, .pdf)
(student version)
- Xplorer GLX-based lab (PDF, 1.3 MB) (1.26 MB, .pdf)
(student version)
Method
Use the CO2 Gas Sensor to directly measure the CO2 gas produced by germinating and dormant seeds.
Here's What You Need
U.S. Educator prices shown.
Probeware
PASPORT Carbon Dioxide Gas Sensor (PS-2110) - $259
Measures carbon dioxide concentration (parts per million) in gases such as air. For use with PASPORT Interfaces.
Xplorer GLX (PS-2002) - $349
The Xplorer GLX is a data collection, graphing, and analysis tool designed for science students and educators.
Other Materials
- Erlenmeyer Flask, 250-mL
- Magnifying Glass
- Protective Gear
- Paper Towel, 1 sheet
- Beans or Pea Seeds (40)
- Water, 100.0 mL
